Coding

Part:BBa_K748001:Design

Designed by: Lei Qiao   Group: iGEM12_HIT-Harbin   (2012-09-15)


AgrC protein coding sequence. AgrC is main component of S.aureus agr quorum sensing system.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

we employed site-specific mutagenesis(AgrC-I A101T) to replace a amino acid residue in AgrC-I so that the mutated AgrC can bound to both AIP-I and AIP-IV.


Source

Staphylococcus aureus genomic sequence

References